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crispr cas9 system  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology crispr cas9 system
    Crispr Cas9 System, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+plasmids/Heme+Oxygenase+1+CRISPR%2FCas9+KO+Plasmid/pm41934679-54-4-12
    Average 94 stars, based on 4 article reviews
    crispr cas9 system - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Knock-Out:

    Article Title: Analysis of the <scp>DNA</scp>‐binding properties of <scp>TGF</scp>‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD3- knockout (A549- S3KO), SMAD2knockout (A549- S2KO), SMAD2/3- double knockout (A549- S2/3DKO), and SMAD4- knockout (A549- S4KO) A549 cells were described previously.14 SMAD2/3/4triple knockout cells (A549- S2/3/4 TKO) were established from A549- S2KO cells using CRISPR plasmids, sc- 400069- NIC- 2 (Santa Cruz Biotechnology) and pDG462- SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′- CTATGCACAATGCTCAGAC- 3′ and 5′- TTGATGTGCCATAGACAAGG- 3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http:// n2t. net/ addge ne: 100903; RRID:Addgene_100903).15 The deletion/disruption of target genes was confirmed by immunoblotting and genomic sequencing (Figure S1). ..

    Article Title: NKG2A Is a Therapeutic Vulnerability in Immunotherapy Resistant MHC-I Heterogeneous Triple-Negative Breast Cancer
    Article Snippet: B2M -KO-IFNγ EMT6 cells were maintained in DMEM-F12 (GIBCO) supplemented with 10% TET-System approved FBS (GIBCO). .. B2M and Qa-1 knockout cell lines were generated using CRISPR plasmids (Santa Cruz) and flow sorted on GFP-positive cells. ..

    Article Title: CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1
    Article Snippet: .. Commercially available CRISPR plasmids (Santa Cruz) were used to alter the expression of CD44 within the hCMEC/D3 cells prior to introduction into the 3D BBB model. To knockout CD44 in the cells, two plasmids encoding a D10A mutated Cas9 nuclease and a CD44-specific 20 nucleotide guide RNA were transfected into cells (Santa Cruz, sc-400209-NIC). ..

    Article Title: CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1
    Article Snippet: .. CD44 knockdown Commercially available CRISPR plasmids (Santa Cruz) were used to alter the expression of CD44 within the hCMEC/D3 cells prior to introduction into the 3D BBB model. To knockout CD44 in the cells, two plasmids encoding a D10A mutated Cas9 nuclease and a CD44-specific 20 nucleotide guide RNA were transfected into cells (Santa Cruz, sc-400209-NIC). ..

    Article Title: NKG2A is a Therapeutic Vulnerability in Immunotherapy Resistant MHC-I Heterogeneous Triple Negative Breast Cancer
    Article Snippet: B2M-KO-IFNγ EMT6 cells were maintained in DMEM-F12 (GIBCO) supplemented with 10% TET-System approved FBS (GIBCO). .. B2M and Qa-1 knockout cell lines were generated using CRISPR plasmids (Santa Cruz) and flow sorted on GFP-positive cells. ..

    Double Knockout:

    Article Title: Analysis of the <scp>DNA</scp>‐binding properties of <scp>TGF</scp>‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD3- knockout (A549- S3KO), SMAD2knockout (A549- S2KO), SMAD2/3- double knockout (A549- S2/3DKO), and SMAD4- knockout (A549- S4KO) A549 cells were described previously.14 SMAD2/3/4triple knockout cells (A549- S2/3/4 TKO) were established from A549- S2KO cells using CRISPR plasmids, sc- 400069- NIC- 2 (Santa Cruz Biotechnology) and pDG462- SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′- CTATGCACAATGCTCAGAC- 3′ and 5′- TTGATGTGCCATAGACAAGG- 3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http:// n2t. net/ addge ne: 100903; RRID:Addgene_100903).15 The deletion/disruption of target genes was confirmed by immunoblotting and genomic sequencing (Figure S1). ..

    CRISPR:

    Article Title: Analysis of the <scp>DNA</scp>‐binding properties of <scp>TGF</scp>‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD3- knockout (A549- S3KO), SMAD2knockout (A549- S2KO), SMAD2/3- double knockout (A549- S2/3DKO), and SMAD4- knockout (A549- S4KO) A549 cells were described previously.14 SMAD2/3/4triple knockout cells (A549- S2/3/4 TKO) were established from A549- S2KO cells using CRISPR plasmids, sc- 400069- NIC- 2 (Santa Cruz Biotechnology) and pDG462- SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′- CTATGCACAATGCTCAGAC- 3′ and 5′- TTGATGTGCCATAGACAAGG- 3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http:// n2t. net/ addge ne: 100903; RRID:Addgene_100903).15 The deletion/disruption of target genes was confirmed by immunoblotting and genomic sequencing (Figure S1). ..

    Article Title: NKG2A Is a Therapeutic Vulnerability in Immunotherapy Resistant MHC-I Heterogeneous Triple-Negative Breast Cancer
    Article Snippet: B2M -KO-IFNγ EMT6 cells were maintained in DMEM-F12 (GIBCO) supplemented with 10% TET-System approved FBS (GIBCO). .. B2M and Qa-1 knockout cell lines were generated using CRISPR plasmids (Santa Cruz) and flow sorted on GFP-positive cells. ..

    Article Title: Analysis of the DNA ‐binding properties of TGF ‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD2/3/4 ‐triple knockout cells (A549‐S2/3/4 TKO) were established from A549‐S2KO cells using CRISPR plasmids, sc‐400069‐NIC‐2 (Santa Cruz Biotechnology) and pDG462‐SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′‐CTATGCACAATGCTCAGAC‐3′ and 5′‐ TTGATGTGCCATAGACAAGG‐3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http://n2t.net/addgene:100903 ; RRID:Addgene_100903). ..

    Article Title: CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1
    Article Snippet: .. Commercially available CRISPR plasmids (Santa Cruz) were used to alter the expression of CD44 within the hCMEC/D3 cells prior to introduction into the 3D BBB model. To knockout CD44 in the cells, two plasmids encoding a D10A mutated Cas9 nuclease and a CD44-specific 20 nucleotide guide RNA were transfected into cells (Santa Cruz, sc-400209-NIC). ..

    Article Title: CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1
    Article Snippet: .. CD44 knockdown Commercially available CRISPR plasmids (Santa Cruz) were used to alter the expression of CD44 within the hCMEC/D3 cells prior to introduction into the 3D BBB model. To knockout CD44 in the cells, two plasmids encoding a D10A mutated Cas9 nuclease and a CD44-specific 20 nucleotide guide RNA were transfected into cells (Santa Cruz, sc-400209-NIC). ..

    Article Title: YAP Overexpression in Breast Cancer Cells Promotes Angiogenesis through Activating YAP Signaling in Vascular Endothelial Cells.
    Article Snippet: For lentiviral particles transduction, Polybrene® (Santa Cruz, USA) reagent was used to introduce retroviral vectors into HUVECs according to manufacturer’s instruction. .. All CRISPR plasmids and shRNA lentiviral particles were purchased from Santa Cruz Biotechnology (Santa Cruz, USA). ..

    Article Title: NKG2A is a Therapeutic Vulnerability in Immunotherapy Resistant MHC-I Heterogeneous Triple Negative Breast Cancer
    Article Snippet: B2M-KO-IFNγ EMT6 cells were maintained in DMEM-F12 (GIBCO) supplemented with 10% TET-System approved FBS (GIBCO). .. B2M and Qa-1 knockout cell lines were generated using CRISPR plasmids (Santa Cruz) and flow sorted on GFP-positive cells. ..

    Article Title: The protective effect of cannabinoids against colorectal cancer cachexia through modulation of inflammation and immune responses.
    Article Snippet: .. According to the manufacturer’s protocol, CRISPR plasmids (Santa Cruz Biotechnology, Dallas, TX, USA) were transfected with UltraCruz Transfection Reagent (Santa Cruz). ..

    Expressing:

    Article Title: Analysis of the <scp>DNA</scp>‐binding properties of <scp>TGF</scp>‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD3- knockout (A549- S3KO), SMAD2knockout (A549- S2KO), SMAD2/3- double knockout (A549- S2/3DKO), and SMAD4- knockout (A549- S4KO) A549 cells were described previously.14 SMAD2/3/4triple knockout cells (A549- S2/3/4 TKO) were established from A549- S2KO cells using CRISPR plasmids, sc- 400069- NIC- 2 (Santa Cruz Biotechnology) and pDG462- SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′- CTATGCACAATGCTCAGAC- 3′ and 5′- TTGATGTGCCATAGACAAGG- 3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http:// n2t. net/ addge ne: 100903; RRID:Addgene_100903).15 The deletion/disruption of target genes was confirmed by immunoblotting and genomic sequencing (Figure S1). ..

    Article Title: Analysis of the DNA ‐binding properties of TGF ‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD2/3/4 ‐triple knockout cells (A549‐S2/3/4 TKO) were established from A549‐S2KO cells using CRISPR plasmids, sc‐400069‐NIC‐2 (Santa Cruz Biotechnology) and pDG462‐SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′‐CTATGCACAATGCTCAGAC‐3′ and 5′‐ TTGATGTGCCATAGACAAGG‐3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http://n2t.net/addgene:100903 ; RRID:Addgene_100903). ..

    Article Title: CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1
    Article Snippet: .. Commercially available CRISPR plasmids (Santa Cruz) were used to alter the expression of CD44 within the hCMEC/D3 cells prior to introduction into the 3D BBB model. To knockout CD44 in the cells, two plasmids encoding a D10A mutated Cas9 nuclease and a CD44-specific 20 nucleotide guide RNA were transfected into cells (Santa Cruz, sc-400209-NIC). ..

    Article Title: CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1
    Article Snippet: .. CD44 knockdown Commercially available CRISPR plasmids (Santa Cruz) were used to alter the expression of CD44 within the hCMEC/D3 cells prior to introduction into the 3D BBB model. To knockout CD44 in the cells, two plasmids encoding a D10A mutated Cas9 nuclease and a CD44-specific 20 nucleotide guide RNA were transfected into cells (Santa Cruz, sc-400209-NIC). ..

    Plasmid Preparation:

    Article Title: Analysis of the <scp>DNA</scp>‐binding properties of <scp>TGF</scp>‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD3- knockout (A549- S3KO), SMAD2knockout (A549- S2KO), SMAD2/3- double knockout (A549- S2/3DKO), and SMAD4- knockout (A549- S4KO) A549 cells were described previously.14 SMAD2/3/4triple knockout cells (A549- S2/3/4 TKO) were established from A549- S2KO cells using CRISPR plasmids, sc- 400069- NIC- 2 (Santa Cruz Biotechnology) and pDG462- SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′- CTATGCACAATGCTCAGAC- 3′ and 5′- TTGATGTGCCATAGACAAGG- 3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http:// n2t. net/ addge ne: 100903; RRID:Addgene_100903).15 The deletion/disruption of target genes was confirmed by immunoblotting and genomic sequencing (Figure S1). ..

    Article Title: Analysis of the DNA ‐binding properties of TGF ‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD2/3/4 ‐triple knockout cells (A549‐S2/3/4 TKO) were established from A549‐S2KO cells using CRISPR plasmids, sc‐400069‐NIC‐2 (Santa Cruz Biotechnology) and pDG462‐SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′‐CTATGCACAATGCTCAGAC‐3′ and 5′‐ TTGATGTGCCATAGACAAGG‐3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http://n2t.net/addgene:100903 ; RRID:Addgene_100903). ..

    Western Blot:

    Article Title: Analysis of the <scp>DNA</scp>‐binding properties of <scp>TGF</scp>‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD3- knockout (A549- S3KO), SMAD2knockout (A549- S2KO), SMAD2/3- double knockout (A549- S2/3DKO), and SMAD4- knockout (A549- S4KO) A549 cells were described previously.14 SMAD2/3/4triple knockout cells (A549- S2/3/4 TKO) were established from A549- S2KO cells using CRISPR plasmids, sc- 400069- NIC- 2 (Santa Cruz Biotechnology) and pDG462- SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′- CTATGCACAATGCTCAGAC- 3′ and 5′- TTGATGTGCCATAGACAAGG- 3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http:// n2t. net/ addge ne: 100903; RRID:Addgene_100903).15 The deletion/disruption of target genes was confirmed by immunoblotting and genomic sequencing (Figure S1). ..

    Genomic Sequencing:

    Article Title: Analysis of the <scp>DNA</scp>‐binding properties of <scp>TGF</scp>‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD3- knockout (A549- S3KO), SMAD2knockout (A549- S2KO), SMAD2/3- double knockout (A549- S2/3DKO), and SMAD4- knockout (A549- S4KO) A549 cells were described previously.14 SMAD2/3/4triple knockout cells (A549- S2/3/4 TKO) were established from A549- S2KO cells using CRISPR plasmids, sc- 400069- NIC- 2 (Santa Cruz Biotechnology) and pDG462- SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′- CTATGCACAATGCTCAGAC- 3′ and 5′- TTGATGTGCCATAGACAAGG- 3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http:// n2t. net/ addge ne: 100903; RRID:Addgene_100903).15 The deletion/disruption of target genes was confirmed by immunoblotting and genomic sequencing (Figure S1). ..

    Generated:

    Article Title: NKG2A Is a Therapeutic Vulnerability in Immunotherapy Resistant MHC-I Heterogeneous Triple-Negative Breast Cancer
    Article Snippet: B2M -KO-IFNγ EMT6 cells were maintained in DMEM-F12 (GIBCO) supplemented with 10% TET-System approved FBS (GIBCO). .. B2M and Qa-1 knockout cell lines were generated using CRISPR plasmids (Santa Cruz) and flow sorted on GFP-positive cells. ..

    Article Title: NKG2A is a Therapeutic Vulnerability in Immunotherapy Resistant MHC-I Heterogeneous Triple Negative Breast Cancer
    Article Snippet: B2M-KO-IFNγ EMT6 cells were maintained in DMEM-F12 (GIBCO) supplemented with 10% TET-System approved FBS (GIBCO). .. B2M and Qa-1 knockout cell lines were generated using CRISPR plasmids (Santa Cruz) and flow sorted on GFP-positive cells. ..

    Triple Knockout:

    Article Title: Analysis of the DNA ‐binding properties of TGF ‐β‐activated Smad complexes unveils a possible molecular basis for cellular context‐dependent signaling
    Article Snippet: .. SMAD2/3/4 ‐triple knockout cells (A549‐S2/3/4 TKO) were established from A549‐S2KO cells using CRISPR plasmids, sc‐400069‐NIC‐2 (Santa Cruz Biotechnology) and pDG462‐SMAD4 (expression for hSpCas9 and 2 gRNAs, 5′‐CTATGCACAATGCTCAGAC‐3′ and 5′‐ TTGATGTGCCATAGACAAGG‐3′). pDG462 was a gift from Prof. Paul Thomas (Addgene plasmid #100903; http://n2t.net/addgene:100903 ; RRID:Addgene_100903). ..

    Transfection:

    Article Title: CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1
    Article Snippet: .. Commercially available CRISPR plasmids (Santa Cruz) were used to alter the expression of CD44 within the hCMEC/D3 cells prior to introduction into the 3D BBB model. To knockout CD44 in the cells, two plasmids encoding a D10A mutated Cas9 nuclease and a CD44-specific 20 nucleotide guide RNA were transfected into cells (Santa Cruz, sc-400209-NIC). ..

    Article Title: CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1
    Article Snippet: .. CD44 knockdown Commercially available CRISPR plasmids (Santa Cruz) were used to alter the expression of CD44 within the hCMEC/D3 cells prior to introduction into the 3D BBB model. To knockout CD44 in the cells, two plasmids encoding a D10A mutated Cas9 nuclease and a CD44-specific 20 nucleotide guide RNA were transfected into cells (Santa Cruz, sc-400209-NIC). ..

    Article Title: The protective effect of cannabinoids against colorectal cancer cachexia through modulation of inflammation and immune responses.
    Article Snippet: .. According to the manufacturer’s protocol, CRISPR plasmids (Santa Cruz Biotechnology, Dallas, TX, USA) were transfected with UltraCruz Transfection Reagent (Santa Cruz). ..

    Knockdown:

    Article Title: CD44 mediates shear stress mechanotransduction in an in vitro blood-brain barrier model through small GTPases RhoA and Rac1
    Article Snippet: .. CD44 knockdown Commercially available CRISPR plasmids (Santa Cruz) were used to alter the expression of CD44 within the hCMEC/D3 cells prior to introduction into the 3D BBB model. To knockout CD44 in the cells, two plasmids encoding a D10A mutated Cas9 nuclease and a CD44-specific 20 nucleotide guide RNA were transfected into cells (Santa Cruz, sc-400209-NIC). ..

    shRNA:

    Article Title: YAP Overexpression in Breast Cancer Cells Promotes Angiogenesis through Activating YAP Signaling in Vascular Endothelial Cells.
    Article Snippet: For lentiviral particles transduction, Polybrene® (Santa Cruz, USA) reagent was used to introduce retroviral vectors into HUVECs according to manufacturer’s instruction. .. All CRISPR plasmids and shRNA lentiviral particles were purchased from Santa Cruz Biotechnology (Santa Cruz, USA). ..



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    Image Search Results


    Video S20. Apical-out organoids-c.705_706insGCAG+Lentivirus

    Journal: Cell Discovery

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia

    doi: 10.1038/s41421-026-00875-8

    Figure Lengend Snippet: Video S20. Apical-out organoids-c.705_706insGCAG+Lentivirus

    Article Snippet: After the primers were annealed, the sgRNA was ligated into the lenti CRISPR V2 vector (Addgene, cat# 52961) using T4 DNA ligase.

    Techniques:

    Video S22. Apical-out organoids-c.-41-2A-C+Lentivirus

    Journal: Cell Discovery

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia

    doi: 10.1038/s41421-026-00875-8

    Figure Lengend Snippet: Video S22. Apical-out organoids-c.-41-2A-C+Lentivirus

    Article Snippet: After the primers were annealed, the sgRNA was ligated into the lenti CRISPR V2 vector (Addgene, cat# 52961) using T4 DNA ligase.

    Techniques: